Seminar: Chandler Day
Identification and resistance to thiophanate-methyl of Botrytis species on Kansas greenhouse crops and a specialty crops grower survey to assess extension IPM resource needs
Gray mold, caused by the fungus Botrytis spp., is a disease that occurs worldwide and affects diverse horticultural crops. Botrytis cinerea, a necrotrophic generalist, is the major cause of gray mold. However, in recent years, other Botrytis species have been identified on an array of crops using molecular diagnostic techniques. The objective of this study was to identify Botrytis species associated with horticultural crops in Kansas and determine sensitivity to methyl benzimidazole carbamate (MBC) fungicides in Kansas strains. Eighty strains were collected from 19 locations, an in vitro assay was conducted to determine sensitivity to fungicides, and strains were identified using three nuclear protein-coding genes (RPB2, G3PDH, and HSP60). Out of eighty strains, seventy-five strains were confirmed as Botrytis cinerea, one clustered with Botrytis pelargonii type specimens, and the other four were inconclusive between Botrytis pelargonii and Botrytis fabae. 78% of strains were highly resistant to thiophanate-methyl, and 95% exhibited at least some resistance. Understanding the species of Botrytis and fungicide sensitivity levels to different active ingredients in Kansas provides growers with science-based information to improve pre- and post-harvest management practices.
Many fruit and vegetable producers grow a wide range of crops with a diverse range of pest problems. To understand and prioritize research and extension needs, 107 fruit and vegetable growers were surveyed about farming systems, experience level, current management, diagnostic capacity, and resource use. Many growers are smaller-scale and less experienced, and they seek information in diverse formats. Both experienced and novice growers’ main diagnostic challenge is disease identification, yet many growers do not use the plant disease diagnostic lab, indicating a need for further training and resources. Our results form a baseline to develop and optimize research and extension priorities to better serve growers. View Abstract (PDF).
Monday, November 18 at 9:00am